Tissue technique collecting in animals
Tissues collected from animals with suspected infectious diseases can be evaluated by several different techniques. Tissue samples should be aseptically placed in appropriate transport media for culture procedures or inoculated into laboratory animals, if indicated, before further handling.
Gently blotting the cut edge of the tissue on a paper towel to remove excess blood and then lightly touching the tissue multiple times to a microscope slide make tissue impressions for cytoligic examination. Tissue specimens can then be frozen, placed into 10% buffered formalin solution, or placed into glutaraldehyde-containing solutions. Frozen specimens are generally superior for immunohistochemical staining and PCR. Routine histopathologic evaluation is performed on formalin-fixed tissues. Special stains can be used to maximize the identification of some infectious agents.
The clinician should alert the histopathology laboratory to to the infectious agents most suspected to allow for appropriate stain selection. Glutaraldehyde-containing fixatives are superior to other fixatives for electron microscopic examination of tissues; this technique can be more sensitive than other procedures for demonstration of viral particles.
We would love to hear your pet's story. Please add a comment.
Gently blotting the cut edge of the tissue on a paper towel to remove excess blood and then lightly touching the tissue multiple times to a microscope slide make tissue impressions for cytoligic examination. Tissue specimens can then be frozen, placed into 10% buffered formalin solution, or placed into glutaraldehyde-containing solutions. Frozen specimens are generally superior for immunohistochemical staining and PCR. Routine histopathologic evaluation is performed on formalin-fixed tissues. Special stains can be used to maximize the identification of some infectious agents.
The clinician should alert the histopathology laboratory to to the infectious agents most suspected to allow for appropriate stain selection. Glutaraldehyde-containing fixatives are superior to other fixatives for electron microscopic examination of tissues; this technique can be more sensitive than other procedures for demonstration of viral particles.
We would love to hear your pet's story. Please add a comment.
MORE VETERINARY ARTICLES
-
The profile of the Golden Retriever may justify for you its reputation as a very good family pet. The Golden Retriever ranks the lowest of a...
-
Clinical signs of acute renal failure symptoms (ARF) are often nonspecific and include lethargy, depression, anorexia, vomiting , diarrhea...
-
Renal efferent glomerular arteriolar constriction, mediated by sympathetic stimulation and angiotensin II, helps maintain glomerular filtrat...
-
The same general mechanisms that cause edema elsewhere in the body cause edema in the pulmonary parenchyma. Major mechanisms are decreased ...
-
Oral Pain 1. Fractured bones or teeth. 2. Trauma. 3. Periodontitis or caries (especially cats). 4. Osteomyelitis. 5. Other causes ...
-
1. Acetaminophen. 2. Antiarrythmics. 3. Anticonvulsants. 4. Antiinflammatories (nonsteroidal). 5. Barbiturates. 6. Benzocaine. 7. Chemothera...
-
Signs and symptoms of heartworm disease in dogs . Many dogs are asymptomatic when the disease is diagnosed by a positive routine screenin...
-
Heartworm prophylaxis is indicated for all dogs living in endemic areas. Because sustained warm, moist conditions are important for heartwor...
-
Feline Leukemia Treatment - Treating Leukemia in Cats Avoiding contact with feline leukemia virus by housing cats indoors is the best form...
-
Clinical heart failure in dogs and cats occurs when the heart is either unable to adequately deliver blood for the body’s metabolic demands...