Tissue technique collecting in animals
Tissues collected from animals with suspected infectious diseases can be evaluated by several different techniques. Tissue samples should be aseptically placed in appropriate transport media for culture procedures or inoculated into laboratory animals, if indicated, before further handling.
Gently blotting the cut edge of the tissue on a paper towel to remove excess blood and then lightly touching the tissue multiple times to a microscope slide make tissue impressions for cytoligic examination. Tissue specimens can then be frozen, placed into 10% buffered formalin solution, or placed into glutaraldehyde-containing solutions. Frozen specimens are generally superior for immunohistochemical staining and PCR. Routine histopathologic evaluation is performed on formalin-fixed tissues. Special stains can be used to maximize the identification of some infectious agents.
The clinician should alert the histopathology laboratory to to the infectious agents most suspected to allow for appropriate stain selection. Glutaraldehyde-containing fixatives are superior to other fixatives for electron microscopic examination of tissues; this technique can be more sensitive than other procedures for demonstration of viral particles.
We would love to hear your pet's story. Please add a comment.
Gently blotting the cut edge of the tissue on a paper towel to remove excess blood and then lightly touching the tissue multiple times to a microscope slide make tissue impressions for cytoligic examination. Tissue specimens can then be frozen, placed into 10% buffered formalin solution, or placed into glutaraldehyde-containing solutions. Frozen specimens are generally superior for immunohistochemical staining and PCR. Routine histopathologic evaluation is performed on formalin-fixed tissues. Special stains can be used to maximize the identification of some infectious agents.
The clinician should alert the histopathology laboratory to to the infectious agents most suspected to allow for appropriate stain selection. Glutaraldehyde-containing fixatives are superior to other fixatives for electron microscopic examination of tissues; this technique can be more sensitive than other procedures for demonstration of viral particles.
We would love to hear your pet's story. Please add a comment.
MORE VETERINARY ARTICLES
-
The strength and regularity of the peripheral arterial pressure waves and the pulse rate are assessed by palpation of the femoral or other p...
-
System venous and right heart filling pressures are reflected at the jugular veins in dogs and cats . These veins should not be distended wh...
-
The precordium in pets is palpated by placing the palm and fingers of each hand on the corresponding side of the animal’s chest wall over t...
-
Pulmonary contusion in dogs and cats is caused by blunt trauma and is a common finding in animals that have been hit by cars. Hemorrhage in...
-
Bacterial infection of the central nervous system (CNS) is rare in dogs. It may result from local extension of infection from adjacent stru...
-
Thoracic auscultation in dogs and cats is used to identify normal heart sounds, determine the presence or absence of abnormal sounds, asses...
-
Brand Name Deramaxx (Novartis) Generic Name Deracoxib (der-ah-cox’-ib) What is the most important information I should know about Der...
-
Signs of cat in heat . The expression " cat in heat " is used by owners to describe the summation of pro-oestrus and oestrus . T...
-
The same general mechanisms that cause edema elsewhere in the body cause edema in the pulmonary parenchyma. Major mechanisms are decreased ...
-
Neoplasms originating from the larynx are uncommon in dogs and cats. More commonly, tumors originating in tissues adjacent to the larynx, su...
